unc5b goat af1006 r d systems Search Results


unc5b goat af1006 r d systems  (R&D Systems)
94
R&D Systems unc5b goat af1006 r d systems
Unc5b Goat Af1006 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pm41703986-145-15-18?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
unc5b goat af1006 r d systems - by Bioz Stars, 2026-08
94/100 stars
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neogenin blocking antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology neogenin blocking antibody
( a ) Migration of human CaSMCs in the presence of <t>recombinant</t> <t>netrin-1.</t> ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of <t>Neogenin,</t> Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.
Neogenin Blocking Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pmc03262880-185-15-18?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 1 article reviews
neogenin blocking antibody - by Bioz Stars, 2026-08
94/100 stars
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anti rabbit igg goat  (Jackson Immuno)
94
Jackson Immuno anti rabbit igg goat
( a ) Migration of human CaSMCs in the presence of <t>recombinant</t> <t>netrin-1.</t> ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of <t>Neogenin,</t> Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.
Anti Rabbit Igg Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pm41703986-145-142-149?v=Jackson+Immuno
Average 94 stars, based on 1 article reviews
anti rabbit igg goat - by Bioz Stars, 2026-08
94/100 stars
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anti rat igg goat  (Jackson Immuno)
93
Jackson Immuno anti rat igg goat
( a ) Migration of human CaSMCs in the presence of <t>recombinant</t> <t>netrin-1.</t> ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of <t>Neogenin,</t> Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.
Anti Rat Igg Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pm41703986-145-128-135?v=Jackson+Immuno
Average 93 stars, based on 1 article reviews
anti rat igg goat - by Bioz Stars, 2026-08
93/100 stars
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anti goat igg rabbit  (Jackson Immuno)
93
Jackson Immuno anti goat igg rabbit
( a ) Migration of human CaSMCs in the presence of <t>recombinant</t> <t>netrin-1.</t> ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of <t>Neogenin,</t> Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.
Anti Goat Igg Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pm41703986-145-114-121?v=Jackson+Immuno
Average 93 stars, based on 1 article reviews
anti goat igg rabbit - by Bioz Stars, 2026-08
93/100 stars
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anti sheep igg donkey  (Jackson Immuno)
91
Jackson Immuno anti sheep igg donkey
( a ) Migration of human CaSMCs in the presence of <t>recombinant</t> <t>netrin-1.</t> ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of <t>Neogenin,</t> Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.
Anti Sheep Igg Donkey, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pm41703986-145-156-163?v=Jackson+Immuno
Average 91 stars, based on 1 article reviews
anti sheep igg donkey - by Bioz Stars, 2026-08
91/100 stars
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horseradish peroxidase hrp conjugated anti mouse igg rabbit  (Jackson Immuno)
92
Jackson Immuno horseradish peroxidase hrp conjugated anti mouse igg rabbit
( a ) Migration of human CaSMCs in the presence of <t>recombinant</t> <t>netrin-1.</t> ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of <t>Neogenin,</t> Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.
Horseradish Peroxidase Hrp Conjugated Anti Mouse Igg Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/unc5b+goat+af1006+r+d+systems/pm41703986-145-97-107?v=Jackson+Immuno
Average 92 stars, based on 1 article reviews
horseradish peroxidase hrp conjugated anti mouse igg rabbit - by Bioz Stars, 2026-08
92/100 stars
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Image Search Results


( a ) Migration of human CaSMCs in the presence of recombinant netrin-1. ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of Neogenin, Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.

Journal: Nature immunology

Article Title: The neuroimmune guidance cue netrin-1 promotes atherosclerosis by inhibiting macrophage emigration from plaques

doi: 10.1038/ni.2205

Figure Lengend Snippet: ( a ) Migration of human CaSMCs in the presence of recombinant netrin-1. ( b ) Migration of CaSMCs in the presence of conditioned media from macrophages treated for the indicated times with LDL or oxLDL (50 μg/ml). ( c ) qPCR analysis of Neogenin, Unc5b and Dcc mRNAs in CaSMC. ( d ) Immunofluorescent staining for neogenin, DCC or isotype-matched control antibody in CaSMCs. Cells were co-stained with DAPI nuclear stain. Scale bar, 10 μm. ( e ) Migration of CaSMCs pre-treated with neogenin or isotype control antibody prior to exposure to conditioned media from macrophages treated as indicated for 24 h. ( f ) Immunofluorescent staining of alpha smooth muscle actin (SMA, green), neogenin (red) and DAPI (blue) in atherosclerotic plaques of Ldlr −/− mice fed a WD. Co-localization of SMA and neogenin (yellow in the merged image) was detected in the media (arrows) and in SMC that had invaded the intima (arrowheads). Staining is representative of plaques from 5 mice. Scale bar, 50 μm. ( a-c, e ) Data are the mean ± s.d. of triplicate samples in a single experiment and are representative of 3 independent experiments. * P < 0.05.

Article Snippet: In some assays, cells were pretreated netrin-1 or UNC5b blocking antibody (AF1006, R&D Systems) or Neogenin blocking antibody (Santa Cruz, sc- 6537) or control goat IgG (Santa Cruz) for 1 h prior to migration.

Techniques: Migration, Recombinant, Staining, Control